A18072 Effect of transplanting adipose tissue-derived mesenchymal stem cells on intimal hyperplasia after carotid balloon injury

Abstract

Objectives: To investigate the effects of transplanting adipose tissue-derived mesenchymal stem cells on intimal hyperplasia after carotid balloon injury and the mechanism Methods: 45 male Sprague Dawley rats were randomly divided into three groups. The balloon catheter was inserted into the left carotid to produce intimal injury. The injured carotid artery and normal carotid artery were used for HE-stained. The mRNA expressions of eNOS were determined by RT-PCR. and the protein expressions of p21, p27 and PCNA were determined by Western Blotting. Serum concentration of NO were detected by ELISA Results: Enhanced green fluorescent protein positive cells were localized to the traumatized arterial segment after transplantation. W/L ratios of ADMSC group were lower than of experimental group. The protein expression of p27, p21 and PCNA and significantly increased after balloon injury, In comparison with injury group, ADMSC treatment significantly increased the protein expression of p27, p21 and decreased PCNA protein expression. The mRNA expressions of eNOS were significantly increased than the experimental group after four weeks treatment, and the serum level of NO were significantly increased after four weeks treatment, compared with experimental group Conclusion: ADMSC could localize to the traumatized arterial segment and inhibit the process of intimal hyperplasia by upregulating the expression of protein p21, p27 and eNOS mRNA, as well as the level of NO in plasma