A17577 Protection of myocardium using seabuckthorn oil in isoproterenol induced myocardial infarction in rats

Abstract

Objectives: Isoproterenol produces weak endogenous antioxidant system, contractile dysfunction, cardiotoxicity & cardiac injury at higher dose. It is reported that catecholamines undergo autoxidation leading to production of free radicals, decreased level of antioxidants in myocardium which is involved in the pathogenesis of cardiovascular diseases such as IR injury. Seabuckthorn oil is widely used in traditional medicine. The present study was aimed to investigate the effect of SBT oil on hemodynamic, biochemical, histopathological & ultrasctructural changes in ISO-induced cardiotoxicity in rats. Methods: Male Wistar albino rats were randomly assigned groups: vehicle treated; SBT oil perse; ISO (85 mg/kg, sc); SBT oil + ISO (3 different doses along with concurrent administration of ISO). Then hemodynamic parameters were evaluated. Animals were sacrificed and hearts were isolated & processed for evaluation of parameters. Results: Systolic, diastolic and mean arterial pressures were significantly reduced in ISO control grp. SBT oil (5–20 mL/kg/d) preserved these parameters in dose-dependent manner. Significant restoration was observed at 10 and 20 mL/kg of SBT oil. ISO control rats showed left ventricular dysfunction as indicated by a significant rise in LVEDP and fall in +LVdp/dtmax values. SBT oil significantly increased LV function. The level of TBARS was significantly increased; the activities of CK-MB and LDH were significantly decreased in heart tissue in ISO control group. SBT oil significantly prevented the rise in activities of CK-MB and LDH, enhanced the content of SOD, catalase, GSH levels. H.E. & ultrastructural studies also showed protection. Conclusion: SBT oil is a safe and effective antioxidant. Thus, a diet containing SBT oil may be beneficial in protecting heart against risk of myocardial damage