Abstract
Objectives: To investigate the effect of D4 dopamine receptor on Na+-K+-ATPase activity in renal proximal tubule (RPT) cells, the underlying mechanism(s) is also determined in this experiment. Methods: Immortalize RPT cells was used in the study to investigate the effect of D4 dopamine receptor agonist PD168077 on Na+-K+-ATPase activity in RPT cells from Wistar-Kyoto (WKY) rats. The NO production were determined by nitrate reductase method. Results: D4 dopamine receptor agonist PD168077 inhibited Na+- K+-ATPase activity in a concentration- and time-dependent manner in WKY RPT cells. The effect was blocked in the presence of D4 receptor antagonist L745870 (10–6 mol/L) and NO synthase inhibitor L-NAME (10–4 mol/L). PD168077 elevated NO production. Conclusion: D4 dopamine receptor agonist PD168077 inhibits Na+-K+-ATPase activity in RPT cells and NO is involved in the mechanisms.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
