A0879 Analysis of structural change of gut microbiota in 2K1C hypertensive rats

Abstract

Objectives: To characterize the gut microbial community structure of two-kidney-one-clip (2K1C) hypertensive rats, providing new ideas for prevention and treatment of hypertension. Methods: The 2K1C hypertensive rat model was established. The total fecal DNA of rats in 2K1C model group and sham operation group were extracted. The structure and principal components of intestinal flora in 2K1C model rats and sham operated rats were then analyzed using Hiseq3000 sequencing platform. Additionally, real time fluorescent quantitative polymerase chain reaction technology was used to detect the content of predominant bacteria, including Escherichia, Bifidobacterium, Lactobacillus and Bacteroides. Results: Compared with sham operation group, significant reduction in the relative abundance of Firmicutes and increases in Bacteroidetes were observed in the fecal samples from model group (P < 0.05). When compared with sham operation group, it also found a significant increase in the relative abundance of Escherichia coli and decrease in the short chain fatty acid producing strains in model group, such as Coprococcus and Roseburia (P < 0.05). Moreover, the relative abundance of Bacteroidetes/Prevotella in model group tended to increase compared with sham operation group (P < 0.05). However, the relative abundance of Firmicutes/Ruminococcus was significant higher than sham operation group (P < 0.05). Principal coordinate analysis showed that there was significant difference in the gut microbial community structure between 2K1C model group and sham operation group. Conclusion: There were significant differences in the gut microbial community structure between 2K1C model group and sham operation group, indicating that gut microbiota may be involved in the pathogenesis of renovascular hypertension in rats.