A0524 Differentially Expressed Genes in Accumulated Epicardial Adipose Tissue Linked to Early Impaired Systolic Cardiac Deformation Ability in a Rat Model of Metabolic Syndrome

Abstract

Objectives: This study aimed to detect early impairment of systolic cardiac function by two-dimensional spackle-tracking strain and strain rate analysis and it's link to epicardial adipose tissue (EAT) in a rat model of metabolic syndrome (MetS). Methods: Sixteen male six-week old SD rats were randomly divided into MetS and control groups. MetS model rats were injected with STZ (15 mg/kg) and fed with high-fat, high-salt diet and water containing 0.02% L-NAME and 10% glucose. All the rats were fed for six weeks. Plasma levels of glucose, insulin and lipids were measured. OGTT and blood pressure measurement were conducted. LVEF was calculated with M-mode echocardiography. Systolic strain and strain rate were analyzed with B-mode echocardiography. Differentially expressed genes in EAT was quantified by genome-wide expression profiling microarray. Results: MetS model rats had higher body weight, blood pressure, plasma glucose and lipids levels (P < 0.05). LVEF, endocardial systolic radial and circumferential strain and strain rate of left ventricle in MetS rats were not different from the controls (P > 0.05). Epicardial systolic radial strain and strain rate in MetS rats were also not different from the controls (P > 0.05). However, epicardial systolic circumferential strain and strain rate in MetS rats were less than the controls (P < 0.05). EAT mass related to impaired epicardial deformation ability negatively (P < 0.01). There were thirty-three differentially-expressed genes in hypertrophic cardiomyo pathway, cardiac muscle contraction pathway, adipo-cytokine signaling pathway or related to heart contraction and lipid storage in EAT in MetS rats. Conclusion: Early impairment of systolic cardiac deformation ability in MetS was possibly myocardial layer-specific. Differentially-expressed genes in EAT possibly played some effects on it.