Abstract
It is a challenge to establish efficient, simplicity and precision miRNA imaging methods in living cells. Herein, we report a ZIF-8 @DNAzyme-based DNA walker strategy for intracellular miRNA imaging with high accuracy. Compared with traditional DNAzyme-based DNA walker, DNAzyme-based DNA walker can be effectively delivered into the cytoplasm by packaging in ZIF-8, thus ensuring the fidelity of intracellular miRNA imaging. In addition, ZIF-8 with acid-degrading properties can be degraded to generate cofactor Zn2+ in the acidic environment of tumors, achieving in the synchronous transport of DNAzyme-based DNA walker and metal ions. This strategy not only displays extremely low background signals in live- cell miRNA imaging, but also can distinguish the expression level of miRNA between tumor cells and normal cells, showing great potential in disease diagnosis. We believe this strategy can further promote the application of DNA-based motors in live-cell miRNA imaging, and provide new ideas for the joint application of MOFs and nucleic acid detection.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
