A widely distributed nuclear protein immunologically related to the microtubule-associated protein MAP1 is associated with the mitotic spindle.

Abstract

A 280-kDa protein (p280) confined to the nucleus of interphase cells becomes associated with the mitotic spindle during cell division. p280 is immunologically related to the microtubule-associated protein MAP1, as shown by cross-reactivity with monoclonal (8D12) and polyclonal antibodies raised against MAP1. However, p280 is distinct from MAP1 as judged by its lower molecular size, proteolytic degradation products, presence in preparations of purified nuclei from which MAP1 is absent, and absence from the cytosol fraction that contains MAP1. Immunofluorescence microscopy of cells in interphase using 8D12 reveals punctate staining of the nucleus, cytoplasmic microtubules, and the microtubule organizing center. Dividing cells display strong staining of the spindle, centrioles, and mid-body. The only exception to this staining pattern is marsupial Pt k2 cells that contain p280 in the nucleus and lack MAP1. These cells exhibit fluorescent staining of the nucleus and the microtubule organizing center when in interphase, of spindle and centrioles in mitosis, and show no staining of cytoplasmic and mid-body microtubules.