Abstract
A highly potent secondary metabolites-producing Bacillus strain was isolated from Mexican soil (Puebla State), together with other fifty strains. The fifty-one strains were subjected for metabolites extraction and evaluated as antibacterial against several bacteria. The active metabolites of these strains were extracted using amberlite XAD16 absorbent resin. The antibacterial activity of crude extracts of all strains was performed by disk diffusion method against some pathogenic gram positive and gram-negative bacteria. Among all Bacillus strains tested, the most potent strain ELI149 (NRB) was selected for molecular characterization. The nucleotide sequence of the 16S rRNA gene (1.5 Kb) of this strain evidenced a 94% similarity with Bacillus amyloliquefaciens strain IIHR-Ba-2, which showed the highest inhibition against the most bacteria probed even greater inhibition than the standard antibiotic. In conclusion, secondary metabolites extracted from Bacillus amyloliquefaciens strain are highly potent as antibiotic against the most bacteria probed. Identification of which metabolites extracted from amberlite are the responsible of the bacteria growth inhibition will be a challenge.
Highlights
A few decades after the introduction of antibiotics into clinical practice, resistance by pathogenic bacteria has become a major health concern
Secondary metabolites play a significant role in clinical practice due to their activity as antimicrobials used in the treatment of microbial induced infections
The gram-negative bacteria used as target to probe the antibiotic activity of the Bacillus extracts were Klebsiella pneumoniae, Serratia marcescens, Escherichia coli, Shigella sp, Salmonella choleraesuis, Vibrio cholerae, Vibrio parahaemolyticus and Pseudomonas aeruginosa; the gram-positive bacteria used as target were
Summary
A few decades after the introduction of antibiotics into clinical practice, resistance by pathogenic bacteria has become a major health concern. Secondary metabolites play a significant role in clinical practice due to their activity as antimicrobials used in the treatment of microbial induced infections. Bacillus species produce secondary metabolites that are the object of natural product chemistry studies. Screening of microbial extracts reveals the large structural diversity of natural compounds with broad biological activities, such as antimicrobial, antiviral, immunosuppressive, and antitumor activities, that enable the bacterium to survive in its natural environment. These findings widen the potential industrial importance of Bacillus spp (SANSINENEA, 2012; SANSINENEA; ORTIZ, 2011). The aim of this study was to evaluate the potential of different bacterial strains to produce biologically active substances with antibacterial activity
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