A wide-field micro-computed tomography detector: micron resolution at half-centimetre scale.

Maksim A Yakovlev,Daniel J Vanselow,Patrick La Riviere,Keith C Cheng,Khai Chung Ang,Mee Siing Ngu,Dula Parkinson,Carolyn R Zaino,Steve Yuxin Wang,Yifu Ding,Spencer R Katz

doi:10.1107/s160057752101287x

Maksim A Yakovlev, Daniel J Vanselow + Show 9 more

Open Access

https://doi.org/10.1107/s160057752101287x

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Abstract

Ideal three-dimensional imaging of complex samples made up of micron-scale structures extending over mm to cm, such as biological tissues, requires both wide field of view and high resolution. For existing optics and detectors used for micro-CT (computed tomography) imaging, sub-micron pixel resolution can only be achieved for fields of view of <2 mm. This article presents a unique detector system with a 6 mm field-of-view image circle and 0.5 µm pixel size that can be used in micro-CT units utilizing both synchrotron and commercial X-ray sources. A resolution-test pattern with linear microstructures and whole adult Daphnia magna were imaged at beamline 8.3.2 of the Berkeley Advanced LightSource. Volumes of 10000 × 10000 × 7096 isotropic 0.5 µm voxels were reconstructed over a 5.0 mm × 3.5 mm field of view. Measurements in the projection domain confirmed a 0.90 µm measured spatial resolution that is largely Nyquist-limited. This unprecedented combination of field of view and resolution dramatically reduces the need for sectional scans and computational stitching for large samples, ultimately offering the means to elucidate changes in tissue and cellular morphology in the context of larger, whole, intact model organisms and specimens. This system is also anticipated to benefit micro-CT imaging in materials science, microelectronics, agricultural science and biomedical engineering.

Highlights

Micro-computed tomography is rapidly becoming a valuable imaging technique for applications where the generation of high-resolution, isotropic, three-dimensional data sets is essential for visualization and both qualitative and quantitative phenotyping (Weinhardt et al, 2018; Hur et al, 2018; Babaei et al, 2016; Seo et al, 2015)
Yakovlev et al A wide-field micro-computed tomography detector camera used in this experiment is an Advanced Photo Systemsized CMOS sensor (CHR70M) with 71 million pixels arranged in 10 000 Â 7096 pixel format with 3.1 mm pixel size, resulting in a 5 mm Â 3.5 mm field of view at 6.2Â magnification (Vieworks VP-71MC produced by Vision Systems Technology)
Yakovlev et al A wide-field micro-computed tomography detector utilize the full field of view of this detector

Summary

Introduction

Micro-computed tomography (micro-CT) is rapidly becoming a valuable imaging technique for applications where the generation of high-resolution, isotropic, three-dimensional data sets is essential for visualization and both qualitative and quantitative phenotyping (Weinhardt et al, 2018; Hur et al, 2018; Babaei et al, 2016; Seo et al, 2015). Micro-CT bridges the gap between resolution, field of view and analytical feasibility by offering isotropic micronscale resolution for mm- to cm-scale samples (Mizutani et al, 2013; Ding et al, 2019). Commercial micro-CT scanners available from companies such as Zeiss, Siemens and Bruker use either commercial flatpanel detectors or microscope objective lenses with field-ofview-to-resolution ratios of about 1000, compared with 10 000 in this work

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