A WICB 50th Favorite: A dynamic actin cytoskeleton functions at multiple stages of clathrin-mediated endocytosis.

Abstract

Molecular Biology of the CellVol. 32, No. 19 ASCB WICB 50th Anniversary FavoriteFree AccessA WICB 50th Favorite: A dynamic actin cytoskeleton functions at multiple stages of clathrin-mediated endocytosisKristen SkruberKristen Skruber*Address correspondence to: Kristen Skruber (E-mail Address: [email protected]).Department of Cellular and Molecular Pharmacology and Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94143Search for more papers by this authorAvital Rodal, Monitoring EditorPublished Online:31 Aug 2021https://doi.org/10.1091/mbc.E21-04-0216AboutSectionsView PDF ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmail To celebrate the 50th anniversary of the American Society for Cell Biology's Women in Cell Biology Committee (WICB), members of WICB and the MBoC Editorial Board invited a diverse group of scientists to highlight MBoC papers by women that have had a scientific or personal impact on the authors of the highlight.While a primary role of actin polymerization is in cellular organization and motility, actin also provides the force and scaffold for trafficking processes such as endocytosis. Actin's role in endocytosis has been challenging to elucidate due to the small scale and transient nature of endocytic structures. Inhibiting actin polymerization in yeast completely halts endocytosis. However, until this landmark paper, the corollary process in mammalian cells was less clear, because ablating actin polymerization only partially stalled trafficking. Yarar et al. (2005) used a mixture of electron and fluorescence microscopy and biochemical methods to establish a clear role for actin polymerization during late stages of clathrin-mediated endocytosis. A clever combination of TIRF and wide-field fluorescence imaging allowed precise vesicle tracking. Importantly, understanding the differences in actin's contributions to endocytosis in yeast and metazoa opened up exciting questions about actin's role in plasma membrane tension and shape.FOOTNOTESDOI: 10.1091/mbc.E21-04-0216REFERENCEYarar D, Waterman-Storer CM, Schmid SL (2005). A dynamic actin cytoskeleton functions at multiple stages of clathrin-mediated endocytosis. Mol Biol Cell 16, 964–975. Link, Google ScholarFiguresReferencesRelatedDetails Vol. 32, No. 19 September 01, 20211793-1866 Metrics Downloads & Citations Downloads: 184 History Information© 2021 Skruber. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).PDF download