Abstract
<p>Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) is broadly used in the food industry for bleaching, sterilization, and deodorization. Detection of H<sub>2</sub>O<sub>2</sub> in food/drinks is important for food safety. In this study, the H<sub>2</sub>O<sub>2</sub>-inducible whole-cell biosensor KT2440 [p<i>PahpC</i>] was constructed based on the bacterial strain <i>Pseudomonas putida</i> KT2440. The H<sub>2</sub>O<sub>2</sub>-inducible promoter <i>PahpC</i> was fused with the reporter gene cluster <i>luxCDABE</i> to produce an H<sub>2</sub>O<sub>2</sub>-inducible bioluminescent biosensor. KT2440 [p<i>PahpC</i>] semi-quantitatively detected H<sub>2</sub>O<sub>2</sub> in the range of 10 - 2000 ?M. This H<sub>2</sub>O<sub>2</sub> biosensor exhibited high specificity and no response to other commonly used redox agents, such as KMnO<sub>4</sub>, Ca(ClO)<sub>2</sub>, and thiourea. This KT2440 [p<i>PahpC</i>] biosensor was used to detect H<sub>2</sub>O<sub>2</sub> in food samples, demonstrating its robust performance. The whole-cell biosensor provides a new approach to the detection of H<sub>2</sub>O<sub>2</sub> in the food industry.</p>
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