A western blot and ELISA assay for the diagnosis of Taenia solium infection in pigs.

Abstract

An enzyme-linked immunoelectrotransfer blot (EITB) was developed using soluble Taenia solium metacestode antigen preparation for immunodiagnosis of porcine cysticercosis and compared with an enzyme-linked immunosorbent assay (ELISA). Sera from 20 pigs with parasitologically confirmed cysticercosis were tested by both EITB and ELISA assays. Specificity of the tests was examined by testing 25 serum samples from the UK, where cysticercosis is not endemic and sera from pigs with Echinococcus granulosus (15), Fasciolopsis buski (six), or Trichinella spiralis (five) infections. All but two of the samples from cases of confirmed cysticercosis were positive by EITB and none of the samples from healthy controls or heterologous infections reacted to any of the diagnostic bands. Thus, the test was 90% sensitive and 100% specific. The sensitivity of the ELISA was 70% with 73% specificity, cross-reactions occurring with sera from E. granulosus infected pigs. Four polypeptides (8, 11, 16 and 23 kDa) were identified by SDS-PAGE and EITB that were specifically recognized by pigs with confirmed cysticercosis.