A visible and colorimetric nanobiosensor based on DNA-CuO nanoparticle for detection of single nucleotide polymorphism involved in sickle cell anemia disease

Abstract

Sickle cell anemia (SCA) is a blood monogenic disease, which is caused by a single nucleotide mutation in the β-globin gene. In this work, to diagnose prenatal of the disease, a colorimetric nanobiosensor established using catalytic and peroxidase-like properties of copper oxide nanoparticle (CuO) and its interaction with DNA. It used to detect SNP in cell-free fetal DNA (cffDNA), as a promising diagnostic biomarker. The nanobiosensor detected the target sequence of the non-target, exactly even with one nucleotide polymorphism. The optimized method was capable of detecting the target sequence over its concentrations ranged from 2 nM to 12 nM (R2 = 0.96) with a detection limit of 0.64 nM within a time of 10 min. To conclude, the SNP detection based on this method shows a cheap, fast, visible, and versatile opportunity in sensitive and selective prenatal diagnosis of hereditary diseases with good reproducibility and stability.