A virus preservation solution that inactivates the virus while maintaining the virus particle intact.

Abstract

The global coronavirus disease 2019 (COVID-19) pandemic has caused immeasurable losses to society. An important part of disease prevention and control is to diagnose infected patients as soon as possible, and nucleic acid detection plays an important role in diagnosing diseases such as COVID-19. Reverse transcription-polymerase chain reaction (RT-PCR) is a common method for the diagnosis of COVID-19 infection, but the false negative rate of covid nucleic acid is currently very high. In this paper, HEK293T cells and lentivirus were used to verify the effect of virus preservation solution, fluorescence method was used to verify the inactivation effect of virus preservation solution, real-time fluorescence PCR experiment was used to verify that the genetic material in virus particles was not destroyed, and protein gel electrophoresis was used to verify the integrity of virus particles in the preservation solution. In this study, we developed a new virus preservation solution using homogeneous reaction technology that can immediately inactivate the virus within 5 minutes at room temperature and maintain the stability of viral nucleic acid for at least 7 days. Importantly, we found that while our solution differed from existing commercial preservatives that contain guanidinium, analyzing the cycle threshold (Ct) showed that it still maintained virion integrity. These advantages could reduce false negatives and the risk of laboratory exposure to infection. In addition, our solution achieved room temperature preservation, which could aid in the transportation of samples. Therefore, this new sample preservation solution could provide significant benefits to the clinical detection of viruses such as COVID-19.