A viral-induced protein in the nuclei of cells infected with fowlpox virus

Abstract

The proteins found in the nuclear and cytoplasmic fractions of fowlpox virus-infected and mock-infected chick embryo cells were examined by SDS-polyacrylamide-gel electrophoresis. At 24 hr PI a viral-induced 37,000-dalton protein was present in infected cytoplasm, and was still being synthesized, although in lesser amounts, at 48 hr. No viral-induced proteins were identified in the nuclear fraction of infected cells at 24 hr PI, but by 36 hr PI a new 36,000-dalton protein had appeared; increased synthesis of this protein was seen at 48 hr PI. There was a general decline in the synthesis of host cell proteins during the period of viral infection.