A Vibrio vulnificus VvpM Induces IL-1β Production Coupled with Necrotic Macrophage Death via Distinct Spatial Targeting by ANXA2

Sei-Jung Lee,Kyung Ku Jang,Kyu-Ho Lee,Ho Jae Han,Jun Sung Kim,Young Hyun Jung,Hyun Jik Lee,Sang Hun Lee,Sang Ho Choi

doi:10.3389/fcimb.2017.00352

Abstract

An inflammatory form of phagocyte death evoked by the Gram-negative bacterium Vibrio (V.) vulnificus (WT) is one of hallmarks to promote their colonization, but the virulence factor and infectious mechanism involved in this process remain largely unknown. Here, we identified extracellular metalloprotease VvpM as a new virulence factor and investigated the molecular mechanism of VvpM which acts during the regulation of the inflammatory form of macrophage death and bacterial colonization. Mutation of the vvpM gene appeared to play major role in the prevention of IL-1β production due to V. vulnificus infection in macrophage. However, the recombinant protein (r) VvpM caused IL-1β production coupled with necrotic cell death, which is highly susceptible to the knockdown of annexin A2 (ANXA2) located in both membrane lipid and non-lipid rafts. In lipid rafts, rVvpM recruited NOX enzymes coupled with ANXA2 to facilitate the production of ROS responsible for the epigenetic and transcriptional regulation of NF-κB in the IL-1β promoter. rVvpM acting on non-lipid rafts increased LC3 puncta formation and autophagic flux, which are required for the mRNA expression of Atg5 involved in the autophagosome formation process. The autophagy activation caused by rVvpM induced NLRP3 inflammasome-dependent caspase-1 activation in the promoting of IL-1β production. In mouse models of V. vulnificus infection, the VvpM mutant failed to elevate the level of pro-inflammatory responses closely related to IL-1β production and prevented bacterial colonization. These findings delineate VvpM efficiently regulates two pathogenic pathways that stimulate NF-κB-dependent IL-1β production and autophagy-mediated NLRP3 inflammasome via distinct spatial targeting by ANXA2.

Highlights

One of the critical activities during bacterial colonization is killing phagocytes recruited by bacterial pathogens at the inflammation site (Fink and Cookson, 2007)
We demonstrate that VvpM mainly targets the critical pathogenic pathway of V. vulnificus to promote IL-1β production coupled with necrotic macrophage death
A key finding of our study is that VvpM acting through annexin A2 (ANXA2) organized into lipid rafts stimulates the Reactive Oxygen Species (ROS)-mediated NF-κB pathway to result in the transcriptional induction of IL-1β, whereas ANXA2 organized into non-lipid rafts induces activation of autophagy and NLRP3 inflammasome, resulting in caspase-1-mediated IL-1β maturation and necrotic macrophage death

Summary

Introduction

One of the critical activities during bacterial colonization is killing phagocytes recruited by bacterial pathogens at the inflammation site (Fink and Cookson, 2007). Vibrio vulnificus is an extremely virulent anaerobic Gram-negative marine bacterium that often causes acute inflammatory responses and the killing of phagocytes in the gut (Toma et al, 2010; Lo et al, 2011). Several other secreted and cell-associated factors have been proposed as potential virulence determinants that are involved in the pathogenesis of V. vulnificus. A 55-kDa zinc-metalloprotease designated as V. vulnificus VvpM is considered to be another major exoprotease that causes cytotoxic effects and an autophagic process affecting intestinal epithelial cells A. et al, 2014, 2015) It remains unclear whether VvpM is a functional virulence factor of V. vulnificus specific to the inflammatory form of phagocyte death with the ability to promote bacterial colonization

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