Abstract
A biochemical assay, only slightly less sensitive than currently used biological assays was developed to detect and quantitate avian oncornaviruses. Using this assay, which is based on the unusual stability of most avian oncornavirus RNA-dependent DNA polymerases at 30°, we were able to quantitate, with an error of ±10%, as few as 1000 Rous sarcoma or Rous-associated virions directly in tissue culture medium. Lower limits of detection for golden pheasant virus and reticuloendotheliosis virus were 3 × 10 4 and 3 × 10 6 virions, respectively. Assay results varied less than fivefold when different strains or subgroups of Rous sarcoma or Rous-associated viruses, having virion-to-infectivity ratios of 7 to 1000, were tested.
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