Abstract
The method includes ethanol intake markers as well as selected metabolites potentially affected by ethanol.
Highlights
The search for answers regarding the effects of alcoholic beverage consumption on human health is hampered by insufficient compliance control during the trials and lack of detailed information about biochemical mechanisms involved in its metabolism and actions in the body
Recent advances in metabolomics research indicate that monitoring of compounds strictly related to speci c food or beverage consumption in biological samples may help to control compliance and understand dietary exposure.[1,2]
This method should allow for simultaneous quanti cation of diverse metabolites affected by alcohol intake or other compounds which can be speci cally present in certain alcohol beverages or affected a er alcohol intake
Summary
The search for answers regarding the effects of alcoholic beverage consumption on human health is hampered by insufficient compliance control during the trials and lack of detailed information about biochemical mechanisms involved in its metabolism and actions in the body. Recent advances in metabolomics research indicate that monitoring of compounds strictly related to speci c food or beverage consumption in biological samples may help to control compliance and understand dietary exposure.[1,2] In order to study the effect of consuming alcohol and related beverages along with the kinetics of consequent metabolite changes, it is essential to develop a versatile analytical method. This method should allow for simultaneous quanti cation of diverse metabolites affected by alcohol intake or other compounds which can be speci cally present in certain alcohol beverages or affected a er alcohol intake. Other possible metabolites to be included in the proposed method such as isoxanthohumol or isocohumulone (putative beer intake biomarkers), 3-nitrotyrosine
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