Abstract
DNA molecules have been utilized both as powerful synthetic building blocks to create nanoscale architectures and as inconstant programmable templates for assembly of biosensors. In this paper, a versatile, scalable and multiplex detection system is reported based on an extending fluorescent resonance energy transfer (FRET) cascades on a linear DNA assemblies. Seven combinations of three kinds of targets are successfully detected through the changes of fluorescence spectra because of the three-steps FRET or non-FRET continuity mechanisms. This nano-assembled FRET-based nanowire is extremely significant for the development of rapid, simple and sensitive detection system. The method used here could be extended to a general platform for multiplex detection through more-step FRET process.
Highlights
Fluorescent resonance energy transfer (FRET) is the most common photophysical process currently investigated, which provides information on the distance between a donor and an acceptor dye in the range of 10 to 100 Å
DNA-based architectures and functional devices are currently playing an increasing role under investigation including molecular scale biosensors[24,25,26,27,28,29,30,31]. Such DNA-based biosensors usually consist of fluorophore quencher pairs and rely on fluorescent resonance energy transfer (FRET), in which distance-dependent fluorescence quenching is elaborately designed to be closely associated with DNA hybridization events
A versatile, scalable and multiplex detection system was reported based on an extending FRET cascades on a linear DNA photonic-wire
Summary
Yansheng Li1, Hongwu Du1, Wenqian Wang[1], Peixun Zhang[2], Liping Xu1, Yongqiang Wen1 & Xueji Zhang[1]. Sequential FRET has been reported by controlling the relative position of multiple fluorophores along a molecular scaffold with demonstrated strategies[13,14,15,16] Inspired by such photonic wires, we expected to design some linear optical sensing devices at nanoscale which could realize multiplex detections simultaneously with high sensitivity and selectivity. Four kinds of dyes (AMCA (D1), FAM (D2), Cy3 (D3) and ROX (D4)) with overlapping absorption and emission spectra (shown in Supporting Information Fig. S1) were modified on the probes and utilized as the photo-energy donor, acceptor or mediator, respectively[37,38,39] Using such platform, seven combinations of three kinds of targets could be successfully detected through the changes of fluorescence spectra before and after the addition of targets. The method could be extended to a general platform for multiplex detection through more-step FRET by attaching more probes with overlapping absorption and emission spectra
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