Abstract
The production of recombinant proteins has helpedinunderstanding of their function and developing newtherapies. However,one of the major bottlenecks for protein production is the establishment of reliable mammalian cell lines with high expression levels. In this chapter, we describe a simple and robust system that allows for the quick establishmentof stable transgenic 293 cell lines withreproducible and high protein expression levels. This methodology is based on the piggyBac transposon system and enablestheinducible production of the protein of interest. Finally,this methodology can easily be used in conventional laboratory cell culture settings without requiring specialized devices.
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