A versatile dual-use RT-PCR control for use in assays for the detection of peste des petits ruminants virus

Abstract

Peste des petits ruminants (PPR) is an acute and highly contagious disease with high mortality in small ruminants and significant socioeconomic impact in developing countries. The causative agent is peste des petits ruminants virus (PPRV). The Food and Agriculture Organization of the United Nations (FAO) and the World Organization for Animal Health (OIE) have set up a goal for the global eradication of PPR by 2030. To assist in this effort, an easily produced, specific, non-pathogenic bacteriophage Qβ based real-time RT-PCR (qRT-PCR) PPRV positive control was developed. This control is compatible for use with two previously described PPRV qRT-PCR assays either as singleplex or multiplex platform. Additionally, the control can also be used for assembling proficiency testing panels for competency testing in diagnostic laboratories. Use of the Qβ phage based PPRV control as a positive control or in proficiency testing panels reduces the risk of inadvertent release of pathogenic PPRV from diagnostic laboratories, which would be especially important should PPR be eradicated in the future.