Abstract
A reverse phase - liquid chromatographic method with UV detection is developed for simultaneous estimation of ceftriaxone sodium and sulbactam sodium in rat plasma. A simple protein precipitation technique was employed for the extraction of drugs from blank plasma. Chromatographic separation of the two drugs was achieved on C18 column (250 mm X 4.6 mm, i.d, 5 μm) using a mobile phase consisting of 10mM potassium dihydrogen orthophosphate buffer (pH - 5) and aceto nitrile (90:10 % v/v). The developed liquid chromatographic method eluted both the drugs with a reasonable retention time, acceptable symmetric peak shape and good resolution. The developed bio - analytical method was linear, accurate, and precise over the co ncentration range of 20 - 150 μg mL - 1 for ceftriaxone and 10 - 75 μg mL - 1 for sulbactam. The developed method was successfully applied to monitor ceftriaxone and sulbactam sodium concentrations in rat plasma.
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