Abstract
This paper presents a RP-HPLC method for the simultaneous estimation of cefixime and cloxacillin in tablets. The process was carried out on C18column (5 μm, 25 cm × 4.6 mm, i.d) using phosphate buffer (pH 5.0), acetonitrile and methanol in the ratio 80:17:3 respectively as a mobile phase at a flow rate of 2mL/min. Wavelength was fixed at 225 nm. The retention time of cefixime and cloxacillin was found to be 5.657 and 6.200 min, respectively. The developed method is rapid and sensitive and it can be used for estimation of combination of these drugs in tablets.
Highlights
Cefixime, chemically designated as (6R, 7R)-7-[2-(2-amino-4-thiazolyl)glyoxylamido]-8-oxo3-vinyl-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid,7-(Z)-[o-(carboxymethyl)-oxime] trihydrate is an antibiotic, and an oral third-generation cephalosporin
From literature survey it was found that various methods have been reported for both the drugs individually but no HPLC methods were reported for such a combination in any type of pharmaceutical dosage form so far
20μL of solution was injected into HPLC system to obtain chromatogram for standard drug solution and sample solution
Summary
Chemically designated as (6R, 7R)-7-[2-(2-amino-4-thiazolyl)glyoxylamido]-8-oxo3-vinyl-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid,7-(Z)-[o-(carboxymethyl)-oxime] trihydrate is an antibiotic, and an oral third-generation cephalosporin. It is highly stable in the presence of beta-lactamase enzymes. Cloxacillin, chemically monosodium (2S, 5R, 6R) – 6 -[ o -(2 -chlorophenyl) – 5 –methyl - 4 –isoxazole carboxamido]-3,3-dimethyl -7-oxo- 4 thia -1- azabicyclo [3.2.0] heptane -2- carboxylate monohydrate is a semisynthetic antibiotic in the same class as penicillin. From literature survey it was found that various methods have been reported for both the drugs individually but no HPLC methods were reported for such a combination in any type of pharmaceutical dosage form so far. The present work describes a simple, precise, and accurate reverse phase HPLC method for simultaneous estimation of Cefixime and Cloxacillin in combined dosage form
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