Abstract
A novel approach was used to develop and validate a rapid, specific, accurate and precise reverse phase high performance liquid chromatographic (HPLC) method for the estimation of Guaifenesin in bulk and pharmaceutical dosage forms. The chromatographic separation was achieved by Cosmosil packed (5C18-MS-II, 4.6 internal diameter 250mm lengths) column, using mobile phase ratio of Acetonitrile: Phosphate buffer PH 5(70:30). The flow rate was 1mL/min and the detection wavelength was 260nm. The limit of detection (LOD) for guaifenesin was 0.23µg/ml and the limit of quantification (LOQ) for guaifenesin was 0.78µg/ml. This method was validated concerning linearity, accuracy, precision, specificity and robustness. The method was also found to be stability-indicating.
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