Abstract
AbstractAn accurate, sensitive, precise and robust reverse phase high performance liquid chromatographic method for the estimation of almotriptan malate in pharmaceutical dosage form has been developed and validated. Chromatographic separation was conducted on Phenomenex Gemini C18 (250 mm × 4.6 mm, 5μ) column at room temperature using potassium dihydrogen phosphate buffer: acetonitrile (80:20) as a mobile phase, pH adjusted to 3.2 ± 0.1 with 10% sodium hydroxide and at a flow rate of 1.5 mL min−1, while UV detection was performed at 227 nm. The retention time for almotriptan malatewas found to be 9.47 ± 0.05 min. The method was found to be linear in the range of 1.0–10.0 μg mL−1. The limit of detection and quantitation for almotriptan malate were found to be 0.03 and 0.1 μg mL−1, respectively. Analytical recovery was > 99.87%. The method was validated stastically and applied for the quantitative analysis of almotriptan malate in bulk and formulations.
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