A Validated Multianalyte LC–MS/MS Method for Quantification of 25 Mycotoxins in Cassava Flour, Peanut Cake and Maize Samples

Abstract

This study was designed to develop a sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the simultaneous detection and quantification of 25 mycotoxins in cassava flour, peanut cake and maize samples with particular focus on the optimization of the sample preparation protocol and method validation. All 25 mycotoxins were extracted in a single step with a mixture of methanol/ethyl acetate/water (70:20:10, v/v/v). The method limits of quantification (LOQ) varied from 0.3 μg/kg to 106 μg/kg. Good precision and linearity were observed for most of the mycotoxins. The method was applied for the analysis of naturally contaminated peanut cake, cassava flour and maize samples from the Republic of Benin. All samples analyzed (fifteen peanut cakes, four maize flour and four cassava flour samples) tested positive for one or more mycotoxins. Aflatoxins (total aflatoxins; 10-346 μg/kg) and ochratoxin A (<LOQ-2 μg/kg) were detected in peanut cake samples while fumonisin B(1) (4-21 μg/kg), aflatoxin B(2) (<LOQ-8 μg/kg), aflatoxin B(1) (<LOQ-9 μg/kg), diacetoxyscirpenol (<LOQ-6 μg/kg) and zearalenone (<LOQ-12 μg/kg) were detected and quantified in cassava flour samples. Fumonisin B(1) (13-836 μg/kg), fumonisin B(2) (5-221 μg/kg), fumonisin B(3) (<LOQ-375 μg/kg) and beauvericin (<LOQ-25 μg/kg) were detected in the maize samples.