Abstract
An LC-MS/MS method was developed and validated for the simultaneous quantitation of dapagliflozin or saxagliptin with metformin in human plasma. Separation and detection of dapagliflozin (DAP), saxagliptin (SAX), metformin (MET) and their internal standards was achieved on Zorbax C18 column (50 × 4.6 mm, 5 μm) using acetonitrile: 0.1% formic acid (45:55, v/v) as a mobile phase applying electrospray ionization (ESI) source in positive ion mode. Sequential liquid-liquid extraction and precipitation techniques were used for the extraction due to the difference in the physicochemical properties of drugs. The MS/MS response was linear over the concentration ranges from 5 to 500 ng/ml, 2–50 ng/ml and 10–4000 ng/ml for DAP, SAX and MET, respectively. Precision, accuracy, selectivity, recovery, and stability were within the acceptable limits as FDA guidelines. The method is suitable for further application of pharmacokinetic studies.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
