Abstract

A new insecticide bioassay for assessing the effects of acute insecticide toxicity on lotic insects was developed. It uses first-instar larvae of a net-spinning caddisfly, Cheumatopsyche brevilineata. The test method was suitable for 30 insecticides with a range of action mechanisms. Caddisfly larvae were much more sensitive than daphnids to neonicotinoids. The new bioassay is thus a useful and reliable method for assessing the impact of chemicals such as neonicotinoids, whose risks for lotic insects might be underestimated by the daphnid bioassay.

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