Abstract
High-sensitivity detection of trace amounts of c-erbB-2 oncogene was reported to be equal to or surpass the ability of CA 15-3 for early diagnosis and/or follow-up recurrent screening of breast cancer. Therefore, in the current study, by using upconversion nanoparticles (UCNPs), rare earth-doped NaYF4:Yb3+/Er3+ as the luminescent labels, a upconversion luminescent (UCL) biosensor based on dual-signal amplification of exonuclease III (ExoIII)-assisted target cycles and long-range self-assembly DNA concatamers was developed for the detection of c-erbB-2 oncogene. The proposed biosensor exhibited ultrasensitive detection with limit as low as 40 aM, which may express the potential of being used in trace analysis of c-erbB-2 oncogene and early diagnosis of breast cancer.
Highlights
To improve the above issue, many strategies based on the enzyme-catalyzed target-recycling signal amplification have been developed and applied for realizing the ultrasensitive DNA detection
Lu group reported a facile approach to prepare DNA-functionalized NaYF4:Yb3+/Er3+ upconversion nanoparticles (UCNPs) based on ligand exchange at the liquid–liquid interface[31], which led to the formation of bioconjugates that retained the characteristic of both DNA and UCNPs
Combining the above methods with previous reports, we would design a new UCL biosensor for the detection of c-erbB-2 oncogene based on dual-signal amplification of exonuclease III (ExoIII)-assisted target cycles and long-range self-assembly DNA concatamers combined with UCNPs
Summary
To improve the above issue, many strategies based on the enzyme-catalyzed target-recycling signal amplification have been developed and applied for realizing the ultrasensitive DNA detection. In order to further improve the sensitivity and selectivity of DNA biosensor, DNA concatamer, one of linear polymeric structures formed by self-association of short DNA fragments through specific interactions, has caught strong attention in DNA diagnostics[13] Their branched 2D or 3D analogs have already been applied to amplify the signal and enhance trapping of the query nucleic acid in hybridization analysis[14]. Combining the above methods with previous reports, we would design a new UCL biosensor for the detection of c-erbB-2 oncogene based on dual-signal amplification of ExoIII-assisted target cycles and long-range self-assembly DNA concatamers combined with UCNPs
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