Abstract
ABSTRACTTo monitor the levels of furaltadone in animal tissues, an immunochromatographic strip (immunostrip) based on quantum dot was developed for rapid detection of 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ), the metabolite of furaltadone. Four monoclonal antibodies (mAbs) against nitrophenyl derivative of AMOZ (2-NP-AMOZ) were generated from the hybridoma cell lines. Among them, the mAb 5C10/3A8 showed the highest sensitivity to 2-NP-AMOZ. Using an immunostrip reader, with the 2-NP-AMOZ as the target, the immunostrip had a liner range from 1 to 30 ng/mL, with 50% inhibition concentration of 5.22 ng/mL and limit of detection of 0.07 ng/mL. The recoveries for AMOZ ranged from 78.6% to 108.9%, with coefficients of variation below 12%. A good correlation (R2 = 0.9901) between the immunostrip and LC–MS/MS results in the tissues tested demonstrated the reliability of the immunostrip. These results indicate that proposed immunostrip is suitable for the rapid and quantitative detection of AMOZ residues in edible animal tissues.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
