Abstract
Cell separation is a critical process in biological and biomedical research, and in clinical therapy. The gold standard of cell separation techniques are fluorescence-activated cell sorting (FACS) and magneticactivated cell sorting (MACS). Despite this, there are still some limitations with FACS and MACS such as low recovery and purity, and affecting the cell physiology and viability. Herein, we report a universal cell separation approach based on reversible binding between N3′-ethyl biotin and streptavidin, which allows for fast capturing of target cells from cell mixture, and fast and complete releasing cells from magnetic beads under mild, physiological conditions. This universal method for detaching cells from magnetic beads provides an efficient cell separation technique based on MACS to give high recovery, high purity, and high viability of cells.
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