Abstract
Azospirillum baldaniorum Sp245 is a well-studied plant growth-promoting bacterium and has recently been shown to possess ∼468 small, non-coding RNAs (sRNA), many of which exhibited differential expression under nutrient stress conditions. Plant-microbe interactions are usually a part of a multigenic response involving a multitude of regulators such as sRNAs and their associated mRNA targets. We report the functional characterization of the sRNA, sSp_p4, from A. baldaniorum Sp245 and confirm its differential expression in nutrient stress conditions. sSp_p4 was overexpressed, knocked down and complemented to characterize its physiological functions and contribution to plant growth promoting (PGP) traits of strain Sp245. Expression analysis of sSp_p4 confirmed that it influenced and regulates PGP traits such as polyhydroxybutyrate synthesis, indole acetic acid production, and biofilm formation. sSp_p4 is likely involved in the regulation of essential bacterial biological networks in this agriculturally relevant bacterial strain and may impact plant growth.
Published Version
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