A twin perspex cell and its associated circuits used to study active transport of sodium across the two halves of a single isolated frogskin when hydrostatic pressures are controlled.

Abstract

A description is given of a twin perspex cell used to study the rate of active transport of sodium across living isolated frogskins in experiments in which the hydrostatic pressures are controlled on each side of the skin. It consists of two half-cells each containing half of the same frogskin; one half-cell acts as a control for the other. A description is given of the circuits used to supply each of the four chambers with oxygenated solution at constant pressure, flow and temperature. The cell is especially suitable for evaluating the effects of small stimuli. It has been used to study the changes in sodium transport brought about by various drugs and hormones and by hydrostatic pressure gradients of less than 3 mm H2O applied across the frogskin.