Abstract

A simple optical method is suggested for the quantitative detection of putrescine using a thiazole derivative (4CTP). The protocol is based on the formation of a complex between putrescine and the 4CTP with ratio 1:2. Synthesized organic compound (4CTP) shows high selective detection of putrescine in various fish samples through the photophysical properties using UV and fluorescence spectroscopy. At the optimal condition, good linearity has been achieved at putrescine concentrations ranging from 5 to 30 μM with low detection limit (LOD) of 68 nM. The binding mechanism is ICT and electronic properties were studied using density functional theory (DFT).

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