A Turn-On Fluorescent Sensor for Glutathione Based on Bovine Serum Albumin-Stabilized Gold Nanoclusters.

Yan Qiu,Jianlin Huang,Li Jia

doi:10.1155/2018/1979684

Abstract

A fluorescence sensor for the detection of glutathione based on the fluorescence recovering of the bovine serum albumin-stabilized gold nanoclusters is reported. This study indicates that glutathione restores the copper-ion-quenched fluorescence by coordinating the bound copper ion in the bovine serum albumin molecule used for stabilizing the gold nanocluster through its sulfydryl. Under the experimental conditions, the fluorescence response showed a linear relationship with the concentration of glutathione over the range from 10 µM to 400 µM. The fluorescence sensor successfully detected glutathione in commercial drug products.

Highlights

Development of glutathione (GSH) assay methods has received attention due to its diverse functions in organisms and extensive market prospects
It was further observed that the AuNCs-bovine serum album (BSA) solution containing Cu2+ emitted strong fluorescence again after treatment with of 1.6 mM GSH (Figure 1)
The fluorescence quenching and recovering efficiency are represented with F0/F1 and F2/F1 respectively, where F0 and F1 correspond to the fluorescence intensity of the AuNCs-BSA in the absence and presence of Cu2+, respectively

Summary

Introduction

Development of glutathione (GSH) assay methods has received attention due to its diverse functions in organisms and extensive market prospects. Based on its essential role in the health of organisms, GSH is used in clinic to treat kinds of diseases such as liver disease and uremia and reduce the side effects correlated with chemoradiotherapy. Many analytical methods, such as high performance liquid chromatography, capillary electrophoresis, fluorophotometry, and electrochemistry, have been developed for detection of GSH [5,6,7,8]. Xie’s research group developed a simple label-free method for the selective and sensitive detection of Hg2+ based on fluorescence quenching of AuNCs-BSA triggered by Hg2+ Au+ interactions [14]. Sensor for GSH based on the AuNCs-BSA-Cu system

Materials and Methods

Results and Discussion

Conclusions