Abstract
A turn-on fluorometric probe is designed for the determination of ascorbic acid (AA) based on the inner filter effect (IFE) between CaF2:[Formula: see text], [Formula: see text] upconversion nanoparticles (CaF2:[Formula: see text], [Formula: see text] NPs) and the oxidized 3, [Formula: see text], 5, [Formula: see text]-tetramethylbenzidine (oxTMB). The colorless TMB can be oxidized by H2O2/[Formula: see text] to blue oxTMB. Under 980 nm photoexcitation, CaF2:[Formula: see text], [Formula: see text] NPs can emit red light, which can be absorbed by the blue oxTMB, leading to the fluorescence quenching at 657 nm. As AA can reduce the blue oxTMB to colorless TMB, the fluorescence at 657 nm can be recovered with the addition of AA, which can accomplish the detection towards AA. The linear detection range of AA is from 1 to 30 [Formula: see text]M, and the limit of detection (LOD) of AA is 0.1 [Formula: see text]M.
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