Abstract
AbstractA novel fluorescence turn‐on assay for heparin has been developed based on DNA‐templated gold nanoclusters (AuNCs) and Cytochrome c (Cyt c). In the design, poly A (A15) ssDNA was selected as a template for fabricating AuNCs. AuNCs possess strong fluorescence emission at 471 nm. Upon addition of Cyt c, the fluorescence of AuNCs was quenched effectively by electron transfer (ET) between AuNCs and the heme cofactor of Cyt c. In the presence of heparin, the much higher electrostatic binding of heparin to Cyt c caused Cyt c to be away from AuNCs, leading to a recovery of fluorescence intensity of AuNCs. The proposed method was found to be simple, fast, and sensitive for heparin detection, with a linear range from 0.5 μg/mL to 12 μg/mL, and a detection limit of 0.15 μg/mL. Furthermore, this work was successfully applied to detection of heparin in fetal bovine serum samples, with good recoveries from 93.53% to 103.6%.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.