Abstract
BackgroundTLPs (Tubby-like proteins) are widespread in eukaryotes and highly conserved in plants and animals. TLP is involved in many biological processes, such as growth, development, biotic and abiotic stress responses, while the underlying molecular mechanism remains largely unknown. In this paper we characterized the biological function of cucumber (Cucumis sativus L.) Tubby-like protein 8 (CsTLP8) in Arabidopsis.ResultsIn cucumber, the expression of the tubby-like protein CsTLP8 was induced by NaCl treatment, but reduced by PEG (Polyethylene Glycol) and ABA (Abscisic Acid) treatment. Subcellular localization and transcriptional activation activity analysis revealed that CsTLP8 possessed two characteristics of classical transcription factors: nuclear localization and trans-activation activity. Yeast two-hybrid assay revealed interactions of CsTLP8 with CsSKP1a and CsSKP1c, suggesting that CsTLP8 might function as a subunit of E3 ubiquitin ligase. The growth activity of yeast with ectopically expressed CsTLP8 was lower than the control under NaCl and mannitol treatments. Under osmotic and salt stresses, overexpression of CsTLP8 inhibited seed germination and the growth of Arabidopsis seedlings, increased the content of MDA (Malondialdehyde), and decreased the activities of SOD (Superoxide Dismutase), POD (Peroxidase) and CAT (Catalase) in Arabidopsis seedlings. Overexpression of CsTLP8 also increased the sensitivity to ABA during seed germination and ABA-mediated stomatal closure.ConclusionUnder osmotic stress, CsTLP8 might inhibit seed germination and seedling growth by affecting antioxidant enzymes activities. CsTLP8 acts as a negative regulator in osmotic stress and its effects may be related to ABA.
Highlights
TLPs (Tubby-like proteins) are widespread in eukaryotes and highly conserved in plants and animals.TLP is involved in many biological processes, such as growth, development, biotic and abiotic stress responses, while the underlying molecular mechanism remains largely unknown
CsTLP8 acts as a negative regulator in osmotic stress and its effects may be related to abscisic acid (ABA)
The results suggest that CsTLP8 can interact with CsSKP1a and CsSKP1c (Fig. 9), indicating that CsTLP8 may function as an F-box protein, a subunit of SCF complex and participate in the degradation of the target protein
Summary
TLPs (Tubby-like proteins) are widespread in eukaryotes and highly conserved in plants and animals.TLP is involved in many biological processes, such as growth, development, biotic and abiotic stress responses, while the underlying molecular mechanism remains largely unknown. TLPs (Tubby-like proteins) are widespread in eukaryotes and highly conserved in plants and animals. In this paper we characterized the biological function of cucumber (Cucumis sativus L.) Tubby-like protein 8 (CsTLP8) in Arabidopsis. TLPs contain an about 270-amino acid tubby domain at the COOH-terminal, with a structure containing 12 anti-parallel β barrels and an intermediate hydrophobic α helix [4]. In Arabidopsis and wheat, AtTLPs and TaTULPs have been confirmed to interact with specific SKP1-like proteins [7,8,9,10], these findings indicate that plant TLPs might function as subunits of SCF complexes. Tubby-like proteins constitute a unique family of transcriptional regulators [4], as the COOH-terminal region of Tubby binds double-stranded DNA, and the
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