A Tos17 transposon insertion in OsCesA9 causes brittle culm in rice

Abstract

The mechanical strength of rice stalks plays an important role in rice growth and development. In this study, the brittle culm mutant bc26 was identified from a tissue culture-derived line of the japonica rice cultivar 'ZH15'. bc26 plants showed fragile leaves and stalks and reduced plant height and spike number compared with wild-type 'ZH15'. Analysis of cell wall components revealed that the cellulose content of stems, leaves, roots and spikes of bc26 plants was significantly lower than that of the wild type, while the hemicellulose content in these tissues of bc26 plants was significantly higher than that of the wild type. Further sequencing using a mixed pool bulked segregant analysis sequencing (BSA-Seq) of brittle plants from the F2 populations localized the bc26 gene to chromosome 9, and a Tos17 transposon insertion in the bc6 gene near the highest SNP-index point was associated with a loss of gene function. Therefore, the bc26 gene was tentatively identified as a new allele of the Bc6 gene, resulting in the brittle culm trait caused by the insertion of the Tos17 transposon.