Abstract
BackgroundParagonimiasis is one of the foodborn trematodiases and number of the patients was estimated to be about 23 million around the world. To obtain good compliance of people for the surveillance of paragonimiasis, an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of paragonimiasis with unconcentrated urine samples was developed.ResultsParagonimus westermani antigen specific IgG and IgG4 were detected in urine samples from paragonimiasis patients and the levels correlated well with those detected in the paired serum samples. Cross-reactions observed among other trematodiasis and a tuberculosis patient with the antigen specific IgG were much reduced by detecting the antigen specific IgG4; 9.2 % to 2.3 %.Conclusions The ELISA with urine samples, which are collected safely and easily, will be a useful tool for a mass-screening of paragonimiasis.
Highlights
Paragonimiasis is one of the foodborn trematodiases and number of the patients was estimated to be about 23 million around the world
We report an enzyme-linked immunosorbent assay (ELISA) to detect antibodies specific to Paragonimus antigens in urine samples from paragonimiasis patients which will be useful for the survey of the lung fluke infections
Anti-adult worm (AW) antibodies in urine from paragonimiasis patients Anti-AW IgG and IgG4 were examined in urine samples from paragonimiasis patients by the ELISA in 27 totally paired urine and serum samples from 19 paragonimiasis patients
Summary
Paragonimiasis is one of the foodborn trematodiases and number of the patients was estimated to be about 23 million around the world. To obtain good compliance of people for the surveillance of paragonimiasis, an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of paragonimiasis with unconcentrated urine samples was developed. Paragonimiasis is a parasitic disease caused by several lung fluke species in the genus Paragonimus. About 23 million people around the world are estimated to be infected with the lung flukes [10]. Diagnosis of patients with the symptoms is important, active surveillance to find out the endemic foci is necessary for control of the disease [17]. As the symptom of paragonimiasis is not always severe, it will be sometimes difficult to obtain good
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