Abstract
Primary epidermal cell cultures and fibroblasts (1st or 2nd subculture), both isolated from embryonic or newborn mouse skin, were tested for differences in membrane antigens by use of rabbit antimouse sera, developed against mouse lymphatic cells or against epidermal material scraped from adult mouse skin. The antisera against mouse tissues were highly reactive in the mixed hemadsorption technique when tested against epidermal or fibroblastic cells from three mouse inbred strains (C57BL/6, B10LP, C3H). Absorption in vivo of these sera by injecting 0.1ml serum per 25mg mouse body weight i.p. into a mouse of the appropriate strain reduced all activity from the sera when tested against fibroblasts. The absorbed serum against lymphatic tissue was also negative when applied to epidermal cells. The absorbed antiepidermal serum, however, remained highly reactive against epidermal cells from all three strains. This reaction was observed immediately after outgrowth and remained detectable for the following 6-8 days. The epidermal antigen may be used for testing the purity of the isolated cell fractions and might further serve to control the epidermal nature of permanent cell lines of epidermal origin.
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