Abstract
A new type of biosensor is presented for detecting protein-protein interaction in solution phase in time-domain. A fast time resolution was achieved by the diffusion measurement with the transient grating method. This technique allows us to study the inter-protein interaction of short-lived intermediate species. This biosensor possesses many unique merits compared with the traditional biosensors, such as the surface plasmon resonance biosensor, and the merits were discussed. We applied this technique to the detection of various intermolecular interactions of photosensor proteins; such as a reaction intermediate of photoactive yellow protein (PYP) and phototropins. Transient photo-induced association and dissociation of intermediates were detected.
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