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Abstract
A thiocoumarin dye, Probe 1, has been developed for the detection of hypochlorite anion (ClO−) via the desulfurization of thiocoumarin. The heavy atom effect efficiently quenched the fluorescence of Probe 1. Upon the addition of ClO−, Probe 1 was instantaneously oxidized into its oxo form, resulting in a strong yellow fluorescence with an emission maximum at 552 nm and a large Stokes shift (141 nm). This probe exhibited excellent selectivity toward ClO− over other reactive oxygen species, common metal ions and anions. Gratifyingly, the typical desulfurizing agents such as Hg2+ and Ag+ had little interfering effect on the behavior of Probe 1 for the detection of ClO−. This probe dispalyed a low detection limit (1.7 × 10−7 M). Importantly, the application of this probe for the detection of endogenous ClO− in living cells had been successfully demonstrated.
Published Version
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