A thermal extrapolation method for the effective temperatures and internal energies of activated ions

Abstract

The internal energies of dissociating ions, activated chemically or collisionally, can be estimated using the kinetics of thermal dissociation. The thermal Arrhenius parameters can be combined with the observed dissociation rate of the activated ions using k diss = A thermalexp(− E a,thermal/ RT eff). This Arrhenius-type relation yields the effective temperature, T eff, at which the ions would dissociate thermally at the same rate, or yield the same product distributions, as the activated ions. In turn, T eff is used to calculate the internal energy of the ions and the energy deposited by the activation process. The method yields an energy deposition efficiency of 10% for a chemical ionization proton transfer reaction and 8–26% for the surface collisions of various peptide ions. Internal energies of ions activated by chemical ionization or by gas phase collisions, and of ions produced by desorption methods such as fast atom bombardment, can be also evaluated. Thermal extrapolation is especially useful for ion–molecule reaction products and for biological ions, where other methods to evaluate internal energies are laborious or unavailable.