A Theoretical Study of the Phase Transition in the Binding of DNA with Mithramycin

Abstract

In the present investigation, we report the theoretical analysis of mithramycin A (MTA) and mithramycin SK (MSK) binding to salmon testes DNA utilizing a modified Zimm and Bragg theory to explain the melting behavior and heat capacity of the DNA before and after drug binding. The sensitivity parameter and half width were employed in tandem to analyze the melting transition’s sharpness. According to the results, a number of metrics, including transition profile, sharpness of transition, heat capacity curve, and half widths, were in good agreement with the experimental observations. The development of novel medications would be made easier by having a better understanding of the molecular interactions between pharmaceuticals and DNA. This theoretical investigation brings us a step closer to the objective of comprehending the stability of nucleic acid interactions with medications and has potential applications in the biomedical industry.