Abstract
Although the biosynthetic pathway of Neocarazostatin A (1) has been identified, the detailed enzymatic reactions underlying the assembly of the carbazole ring still remain largely unknown. We demonstrate here that NzsH, a putative thiamine diphosphate dependent enzyme, can catalyze an acyloin coupling reaction between indole-3-pyruvate and pyruvate to generate a β-ketoacid intermediate. Our findings thus shed light on further characterization of the unusual biosynthetic pathway of the bacterial tricyclic carbazole alkaloids.
Highlights
Carbazoles are a group of organic compounds consisting of a tricyclic nucleus with two benzene rings flanking a pyrrole ring.[1]
We have demonstrated the essential role played by NzsH in the biosynthesis of 1 by showing that the production of 1 was completely abolished in the ΔnzsH mutant (Fig. 1B)
Communication against free radicals, and for the intriguing enzymatic reactions underlying the assembly of the carbazole ring
Summary
Carbazoles are a group of organic compounds consisting of a tricyclic nucleus with two benzene rings flanking a pyrrole ring.[1].
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