Abstract
Background: TGFβ is an important regulator of growth and differentiation in pancreas. We have recently demonstrated that TGFβ activates PKA in a mink lung epithelial cell model through complex formation of Smads with PKA independent of increases in cAMP (Mol Cell Biol 2004; 24: 2169-2180). In this study, we sought to evaluate whether TGFβ can activate PKA in pancreatic acinar cells, the mechanism by which PKA is activated, and PKA’s role in TGFβ-mediated pancreatic growth regulation. Methods: Isolated mouse pancreatic acini were treated with TGFβ and in vitro kinase assays for PKA activity were performed using a biotinylated PKA peptide substrate.
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