A test of the vicariance hypothesis of western North American freshwater biogeography

Abstract

AbstractAim The biogeography of western North American freshwater molluscs has traditionally been attributed to vicariance associated with late Tertiary rearrangement of landscape based on distributional evidence and the putatively limited dispersal ability of these organisms. We examined the phylogeography of a widely ranging western springsnail (Pyrgulopsis wongi Hershler, 1989) to test this hypothesis and evaluate the relative importance of vicariance and dispersal in structuring the distribution of this species.Location Southwestern Great Basin (California and Nevada), United States of America.Method Two mitochondrial genes (COI, NDI) were sequenced for 28 populations of P. wongi spanning its entire geographic range, which consists of 10 topographically closed drainage basins. We also sequenced eight closely related congeners, as well as the type species of the closely related eastern North American genus Floridobia Hershler & Thompson, 2002, which was used as the outgroup. Phylogenies based on the combined data set were obtained using several methods, and networks for each gene were generated as an additional means of examining relationships among haplotypes. Partitioning of haplotype variation was studied using amova, migration between populations was estimated using a coalescent‐based method (mdiv), and divergence times were inferred using a locally calibrated molecular clock and mdiv.Results Pyrgulopsis wongi is subdivided into narrowly localized and widely distributed lineages that diverged in the Pleistocene, well after the inception of the contemporary regional landscape. While large ΦST values and the localized geographic distributions of most haplotypes imply absence or negligible contemporary dispersal of this spring‐dwelling snail, the pattern of phylogeographic structuring, presence of a few widespread haplotypes, and results of the mdiv analyses suggest geologically recent dispersal across drainage divides.Main conclusions Phylogeography of P. wongi conflicts with the traditional vicariance model as it is not structured by the contemporary landscape and is instead indicative of geologically recent dispersal. In the absence of evidence that dispersal of this species occurred through surface water connections during the relevant (Quaternary) time frame, we conjecture that spread may have instead been mediated by transport on waterfowl or via upland stream capture. The non‐concordance between phylogeography and landscape reported in this and other recent studies of Pyrgulopsis suggests that members of this diverse and imperiled genus should not be managed using an a priori, ‘watershed as conservation unit’ approach.