A Test of the Excitability-Gradient Hypothesis in the Swimmeret System of Crayfish

Abstract

The motor pattern that drives coordinated movements of swimmerets in different segments during forward swimming characteristically begins with a power-stroke by the most posterior limbs, followed progressively by power-strokes of each of the more anterior limbs. To explain this caudal-to-rostral progression, the hypothesis was proposed that the neurons that drive the most posterior swimmerets are more excitable than their more anterior counterparts, and so reach threshold first. To test this excitability-gradient hypothesis, I used carbachol to excite expression of the swimmeret motor pattern and used tetrodotoxin (TTX), sucrose solutions, and cutting to block the flow of information between anterior and posterior segments. I showed that the swimmeret activity elicited by carbachol is like that produced when the swimmeret system is spontaneously active and that blocking an intersegmental connective uncoupled swimmeret activity on opposite sides of the block. When anterior and posterior segments were isolated from each other, the frequencies of the motor patterns expressed by anterior segments were not slower than those expressed by posterior segments exposed to the same concentrations of carbachol. This result was independent of the concentration of carbachol applied and of the number of segmental ganglia that remained connected. When TTX was used to block information flow, the motor patterns produced in segments anterior to the block were significantly faster than those from segments posterior to the block. These observations contradict the predictions of the excitability-gradient hypothesis and lead to the conclusion that the hypothesis is incorrect.