Abstract
The standardisation of a terminal labelling cytotoxicity assay which used the incorporation of 3H-uridine to measure target cell survival of the effector phase is described. This assay was applied to a preliminary study of the cell-mediated responses of sheep following intravenous inoculation of Pi-3 virus. The assay provided a simple, accurate, and reproducible method to measure cytotoxic responses against Pi-3 infected target cells. The experimental data did not require mathematical corrections for non-specific effects as in the 51 Cr-release assay. When cytotoxicity could be detected in uninfected target cell cultures it was low and statistically insignificant
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